O039 Subnormothermic acellular perfusion of human kidneys

Abstract Introduction The optimum conditions for normothermic machine perfusion (NMP) in kidney transplantation have yet to be determined. Traditionally, kidneys are perfused with a red blood cell (RBC) based solution at 37°C. NMP subnormothermic temperature without RBCs could reduce the harmful effects of haemolysis but still preserve function. This study examines effect acellular (SNAP) human kidneys. Methods Five pairs offered research were included study. One each pair was an oxygenated albumin-based cells and other 32°C 6h. After perfusion, reperfused 4h cell-based 37°C assessment. Perfusion parameters renal function compared between groups. Results mean age donors 66±12y. flow (RBF) numerically higher SNAP group (156.5±57.1 vs 115.3±41.21ml/min/100g; P=0.063). RBC produced more urine this did not reach statistical significance (154±159 64±73ml; P=0.217). During reperfusion levels RBF, output percentage creatinine fall similar groups (Creatinine fall; 66±18 74±16%; P=0.382). Conclusion suggests that is equivalent no adverse on parameters. removal negates any detrimental provides simpler method perfusion.